One of the species that emerged from the inventory of Gentiana family is Gentiana olivieri Griseb. Since this species have a particular occurrence in the nature and it’s still subjected to the ignorance and needs special treatment for the defined activity and the mode of action of drug. The plant is found in temperate region of Gulf countries and some temperate region of China. The objective of this paper was to establish chemical profile of Gentiana olivieri Griseb. using preliminary chemical tests and TLC studies. Identification of primary and secondary constituents has become the utmost important tool for the presence of active moiety. The phytochemical screening of petroleum ether, chloroform, acetone, ethanol, methanol and water extracts of Gentiana olivieri Aerial parts was done by chemical tests and TLC studies. Petroleum ether and chloroform extract showed the presence of fatty acids and alkaloids, acetone, methanol and ethanol extracts showed the presence of flavonoids and other phenolic compounds. While ethanol extract showed the presence of triterpenoids and some flavonoids. Ethyl acetate fraction was also prepared separately that showed the presence of biflavones. TLC profiling of above mentioned extracts showed the presence of at least 40 compounds that may be available belonging to different categories, which may be used to exploit the potential of this rare plant.
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